Nuclear localization of ciliary neurotrophic factor in glial cells

In this work we studied the subcellular localization of ciliary neurotrophi c factor (CNTF) in primary culture of rat cortical type I astrocytes and ra t glioma C6 cells, transfected COS-7 cells and in the Xenopus oocytes. in a ll these models, morphological and biochemical evidence are provided for th e nuclear localization of CNTF. In addition the nuclear translocation of CN TF is temperature-sensitive and thus strongly suggestive of a mechanism of facilitated transport. (C) 1999 Elsevier Science B.V. All rights reserved.