Q. Davies et al., An immunoscintigraphic evaluation of the engineered human monoclonal antibody (hCTMO1) for use in the treatment of ovarian carcinoma, BR J OBST G, 106(1), 1999, pp. 31-37
Objective To assess the safety and targeting ability of the engineered huma
n antibody (hCTMO1) in women with ovarian carcinoma.
Design The monoclonal antibody labelled with Indium-111 was administered to
women with suspected primary or recurrent ovarian carcinoma six days pre-o
peratively. The first group of women was given a dose of 0.1 mg per kg body
weight of radiolabelled antibody. A second group of women received 1 mg pe
r kg body weight and finally a third group was given 1 mg per kg body weigh
t of unlabelled antibody followed one hour later by 0.1 mg per kg body weig
ht of radiolabelled antibody. All the women were then imaged using a gamma
camera one hour and up to 96 hours after injection.
Participants Fourty-four women in whom there was a high suspicion of primar
y ovarian carcinoma on the basis of ultrasound or CT imaging and serum CA12
5 and those in whom there was a suspicion of recurrent ovarian carcinoma af
ter being treated for histologically confirmed carcinoma.
Setting The Queen's Medical Centre, Nottingham and University Hospital Vrij
e Universiteit, Amsterdam, The Netherlands.
Results At the low dose of antibody the sensitivity for detection of ovaria
n carcinoma was 70%. After increasing the dose of antibody and also after p
re-dosing with unlabelled antibody the sensitivity increased to 100%, but t
here was a large number of false positive results at the higher dose, and t
herefore the specificity was low. The liver and bone marrow were the organs
with the highest activities.
Conclusion The genetically engineered antibody hCTMO1 is safe for use in wo
men. This antibody effectively targets ovarian carcinoma and has greater po
tential as a vector for therapeutic use than as a diagnostic agent.