Resolution of infrared spectra and kinetic analysis of mutarotation of D-mannose in water

infrared spectra of a solution of D-mannose undergoing mutarotation were ac quired at regular time intervals. The spectra were first-order differentiat ed and sequential rank analysis was used to obtain the concentration profil es of the anomers. Least-squares were then used to resolve the infrared spe ctra of the anomers. Rate constants for the conversion from alpha- to beta- D-mannose were calculated from the concentration profiles. The constants ar e slightly different from earlier reported values, while the equilibrium co ncentrations agree well with reported values. (C) 1999 Elsevier Science B.V . All rights reserved.