Comparative gene mapping: cytogenetic localization of PROC, EN1, ALPI, TNP1, and IL1B in cattle and sheep reveals a conserved rearrangement relative to the human genome

The cytogenetic locations of the genes for protein C (PROC), transition pro tein 1 (TNP1), intestinal alkaline phosphatase (ALPI), engrailed (EN1), and human protointerleukin beta (IL1B) have been compared between cattle (Bos taurus, BTA) and sheep (Ovis aries, OAR). Bovine YAC and cosmid clones were used as FISH probes to determine the order (centromere to telomere) of fou r of these genes on OAR 2q, as well as the location of IL1B on OAR 3p. In c attle, IL1B and EN1 were assigned to BTA 11 and BTA 2, respectively. Alignm ent of the ovine, bovine, and human physical maps based on these data shows that segments of conserved synteny and chromosomal rearrangements detected between cattle and human are also found in sheep, where the order in cattl e is conserved.