A. Leprini et al., Rat tenascin-R gene: structure, chromosome location and transcriptional activity of promoter and exon 1, CYTOG C GEN, 83(1-2), 1998, pp. 115-123
Tenascin-R is an extracellular matrix protein expressed exclusively in the
central nervous system where it is thought to play a relevant role in regul
ating neurite outgrowth. We have i) cloned the cDNA of the rat tenascin-R 5
' region; ii) defined its genomic organization, obtaining the sequence of t
wo novel untranslated exons; iii) mapped the gene to rat chromosome 13q23 a
nd suggested a previously unreported synteny between rat chromosome 13q23,
human chromosome 1q24, and mouse chromosome 4E; and iv) sequenced and chara
cterized the elements responsible for its neural cell-restricted transcript
ion. We found that two discrete regions of the rat gene (the first in the p
roximal promoter, the second in the first exon) are independently able to a
ctivate to a high degree the transcription of a reporter gene in either hum
an or rat neuroblastoma cell lines but not in other cell lines. Based on th
is observation, we reevaluated the arrangement of transcriptionally active
regions in the human tenascin-R gene we recently cloned and found that the
human gene also contains an exon sequence able to initiate and sustain tran
scription independently of promoter sequences.