Evaluation of topical application of clobetasol 17-propionate from variouscream bases

The effect of clobetasol 17-propionate (CP), a potent corticosteroid, in va rious cream bases an the permeation through artificial membrane was sought. Four formulations were then chosen for a further in vivo skin blanching as say. After calculation of the relationship between in vivo flux(0-8hr) dete rmined from a surface recovery technique and in vitro release rate(0-8hr) o f CP from various formulations, a high correlation coefficient of 0.9996 wa s achieved. Therefore, the in vitro release study could be used as an index to predict and evaluate the in vivo penetration capacity of CP cream to sc reen the effective formulation preclinically. After a series of in vivo inv estigations in this study, it was concluded that myristic acid-added formul ations may show a bioequivalence with commercial Dermovate(R). Furthermore, the flux calculated from the surface recovery technique and Delta E* detec ted from the skin blanching assay may be useful as parameters evaluating th e quality and effectiveness of CP cream.