A low-volume electrochemical cell for the deposition of conducting polymers and entrapment of enzymes

A new small-volume electrochemical cell for the entrapment of biological re cognition elements in electrochemically deposited conducting polymer films has been developed. It allows one to save significant amounts of the monome r and especially of the used biological recognition element, and hence perm its one to choose very expensive enzymes for the development of respected b iosensors. Since the conductivity, morphology and film thickness of the obt ained conducting polymer films are dependent on the presence of molecular o xygen, the electrochemical cell allows a facilitated handling of the electr ode and electrolyte solution under strict exclusion of any oxygen. To prove the applicability of the new electrochemical cell, the entrapment of gluco se oxidase into polypyrrole films has been performed as an example.