Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography

Citation
H. Wan et al., Highly efficient separation of isomeric epoxy fatty acids by micellar electrokinetic chromatography, ELECTROPHOR, 20(1), 1999, pp. 132-137
Citations number
15
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
01730835 → ACNP
Volume
20
Issue
1
Year of publication
1999
Pages
132 - 137
Database
ISI
SICI code
0173-0835(199901)20:1<132:HESOIE>2.0.ZU;2-F
Abstract
A capillary electrophoresis (CE) method has been developed for simple and d irect separation of cis- and trans-12,13-epoxy-9(Z)-octadecenoic acid and 9 ,10-epoxy-12(Z)-octadecenoic acid isomers. Separation was performed in mice llar electrokinetic capillary chromatography (MEKC) using a buffer consisti ng of 25 mM berate (pH 9.20), 10 mM sodium dodecyl sulfate (SDS) and 10% v/ v acetonitrile. The key variables, concentrations of SDS and organic modifi er, were optimized by the application of a factorial experimental design. T he use of a low micellar concentration, just above critical micelle concent ration (CMC), in a background electrolyte containing an organic modifier no t only made it possible to dissolve and separate highly hydrophobic fatty a cid isomers, but also resulted In improved separation efficiency and select ivity. Separation efficiency up to 4 x 10(5) theoretical plates/m was achie ved under an optimized condition. Also investigated were the influence of t emperature on separation and the effect of organic modifier concentration o n the dynamic exchange of the analytes between micelles and the bulk of the buffer solution. Direct UV was;applied for detection of the fatty acids.