Chiral resolution of atropine, homatropine and eight synthetic tropinyl and piperidinyl esters by capillary zone electrophoresis with cyclodextrin additives

Chiral resolution of atropine, homatropine and eight synthetic tropinyl and piperidinyl esters were studied by capillary zone electrophoresis with cyc lodextrin additives. Atropine and eight synthetic derivatives were successf ully resolved by heptakis-(2,3,6-tri-O-methyl)-beta-cyclodextrin (TM-beta-C D) at concentrations ranging from 10 to 40 mnn. Homatropine was baseline re solved by 10 mM beta-cyclodextrin and hydroxypropyl-beta-cyclodextrin cyclo dextrin (HP-beta-CD), respectively. The developed method was employed for t he determination of atropine enantiomers in human serum.