Ma. Olsen et Le. Schechter, Cloning, mRNA localization and evolutionary conservation of a human 5-HT7 receptor pseudogene, GENE, 227(1), 1999, pp. 63-69
Initial experiments designed to clone novel serotonin receptor subtypes in
the substantia nigra have led to the discovery of a transcribed human 5-HT7
receptor pseudogene that is expressed in a wide range of tissues. The orig
inal clone (S771) possessed greater than 90% homology to the 5-HT7 receptor
sequence and was identified by a degenerate PCR approach. Expression of th
e pseudogene transcript was detected throughout the brain and peripheral ti
ssues in general agreement with 5-HT7 mRNA localization. interestingly, the
transcript was detected in tissues not known to express the 5-HT7 receptor
(i.e. liver and kidney). Analysis of genomic DNA explained the genesis of
the human pseudogene via a processed parental transcript (retrotranspositio
n) and led to the discovery of a species homologue in the rhesus monkey. (C
) 1999 Elsevier Science B.V. All rights reserved.