Block matching is a valuable tool for selecting donors for bone marrow tran
splantation. Identical, electrophoretic profiles of unrelated bone marrow d
onor-recipient pairs have been shown to be associated with long-term surviv
al and a reduction of graft versus host disease (GVHD). This study was unde
rtaken to determine the sequences of the PCR products which are generated.
PCR products obtained with beta-block primers following the amplification o
f DNA extracted from cell lines homozygous for 7.1 and 8.1 ancestral haplot
ypes were cloned and sequenced. The PCR products were characterised and the
beta block profiles reconstructed. The data indicate that the profiles con
sist of homoduplexes and heteroduplexes which are formed by the products of
probably ? different sequence locations. Human Immunology GO, 171-176 (199
9). (C) American Society for Histocompatibility and Immunogenetics, 1999. P
ublished by Elsevier Science Inc.