Reconstruction of the block matching profiles

Citation
N. Ketheesan et al., Reconstruction of the block matching profiles, HUMAN IMMUN, 60(2), 1999, pp. 171-176
Citations number
15
Categorie Soggetti
Immunology
Journal title
HUMAN IMMUNOLOGY
ISSN journal
01988859 → ACNP
Volume
60
Issue
2
Year of publication
1999
Pages
171 - 176
Database
ISI
SICI code
0198-8859(199902)60:2<171:ROTBMP>2.0.ZU;2-9
Abstract
Block matching is a valuable tool for selecting donors for bone marrow tran splantation. Identical, electrophoretic profiles of unrelated bone marrow d onor-recipient pairs have been shown to be associated with long-term surviv al and a reduction of graft versus host disease (GVHD). This study was unde rtaken to determine the sequences of the PCR products which are generated. PCR products obtained with beta-block primers following the amplification o f DNA extracted from cell lines homozygous for 7.1 and 8.1 ancestral haplot ypes were cloned and sequenced. The PCR products were characterised and the beta block profiles reconstructed. The data indicate that the profiles con sist of homoduplexes and heteroduplexes which are formed by the products of probably ? different sequence locations. Human Immunology GO, 171-176 (199 9). (C) American Society for Histocompatibility and Immunogenetics, 1999. P ublished by Elsevier Science Inc.