Bio-stretch, a computerized cell strain apparatus for three dimensional organotypic cultures

In the present study, a unique mechanical strain apparatus for three-dimens ional organotypic cultures was developed into a computerized system. It con sists of a personal computer running Windows-based software, the Bio-Stretc h Manager, a Bio-Stretch Controller, and three sets of magnet boards. Cells are cultured on a Gelfoam(R) sponge that is placed in a 35-mm petri dish w ith one end glued to the dish, and the other end attached to a coated steel bar. The petri dish is placed in front of a magnet, and the movement of th e steel bar is controlled by dynamically changing the magnetic field. Up to five stretch patterns of variable frequency; duty cycle. and magnitude can be designed for each stretch regimen. Three different stretch regimens can be tested simultaneously. The operational characteristics of sponges were examined. Attachment of cells to the sponges was observed on several cell t ypes. These features provide wide options for using this system to study th e effects of mechanical stretch on cells.