Effects of the indigenous microflora of minimally processed lettuce on thesurvival and growth of Listeria innocua

Interactions between the natural background microflora of shredded lettuce and Listeria innocua (in lieu of Listeria monocytogenes) were studied. The effect of increasing the initial size of indigenous populations (from 10(3) to 10(6)-10(7) CFU g(-1)) was tested for its ability to reduce L. innocua growth on shredded lettuce. Co-culture experiments were performed in model media, where bacterial isolates from the indigenous microflora were tested for possible inhibitory effects. Varying the size of the indigenous populat ions had no effect on L. innocua survival or growth. However, interactions with individual species and mixed populations from lettuce did affect the s urvival and growth of L. innocua in model media. In general, mixed populati ons diminished L. innocua growth. In the undiluted lettuce medium, the vari ous species tested individually either reduced or did not affect the growth of L, innocua. However, when the medium was diluted, some species extended the survival of L, innocua. Competition between the indigenous microflora and L, innocua resided mostly with the Enterobacter spp. and not with the p seudomonads. Enterobacter cloacae was particularly effective in reducing L. innocua growth. Lactic acid bacteria also reduced L. innocua growth in und iluted media. It is concluded that interactions with the natural background microflora may play an important role in determining the dynamics of Liste ria populations on shredded lettuce.