Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

Authors
Pouget, JP Ravanat, JL Douki, T Richard, MJ Cadet, J
Citation
Jp. Pouget et al., Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation, J CHIM PHYS, 96(1), 1999, pp. 143-146
Citations number
4
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE → ACNP
Volume
96
Issue
1
Year of publication
1999
Pages
143 - 146
Database
ISI
SICI code
Abstract
We used the comet assay associated with DNA-glycosylases to estimate DNA da mage in cells exposed to gamma irradiation or photosensitized either with m ethylene blue or orange acridine. A calibration performed using gamma irrad iation allowed the measurement of the steady-state level and the yield of 8 -oxodGuo as well as strand breaks and alkali-labile sites.