Validation of thin-layer chromatography quantitation determination with CCD camera and slit-scanning densitometer

Video densitometric and classical slit-scanning determinations of pesticide s on thin-layer chromatographic plates were validated for linearity, precis ion, and detection limit, A comparison of results showed that slit-scanning densitometry is more sensitive and more precise than video densitometry. H owever, according to validation requirements, relative standard deviation f or video densitometry is acceptable (3.5-5.3%). Linearities are very good a nd almost identical for both techniques. The main advantage of video techno logy is speed (a few seconds, compared with 20 min with scanning densitomet ry). Video technology also provides excellent archiving facility. Image and chromatographic data can be stored together, edited, and used for many tas ks.