Reversed-phase thin-layer chromatography with different stationary phases in studies of quantitative structure-biological activity relationship of new antimycotic compounds

Reversed-phase thin-layer chromatography with RP-8, RP-18, and RP-18W stati onary phases was used in quantitative structure-activity relationship (QSAR ) studies of new antimycotic compounds. The retention behavior of 10 dihydr oxythiobenzanilides was examined for acquisition of log K data. With water- acetone mixtures as the mobile phases, the concentration range for which th e correlation between log K and acetone concentration is linear was establi shed for each stationary phase and used to determine hydrophobicity paramet ers log K-w by linear extrapolation, The effect of substituents on retentio n constants was quantitated by using the group contribution parameters tau( w). On the basis of OSAR equations obtained from these studies, log K-w dat a can be used to predict antifungal activities of dihydroxythiobenzanilides with satisfactory accuracy.