Ca2+-induced Ca2+ release in chromaffin cells seen from inside the ER withtargeted aequorin

The presence and physiological role of Ca2+ induced Ca2+ release (CICR) in nonmuscle excitable cells has been investigated only indirectly through mea surements of cytosolic [Ca2+] ([Ca2+](c)). Using targeted aequorin, we have directly monitored [Ca2+] changes inside the ER ([Ca2+](ER)) in bovine adr enal chromaffin cells. Ca2+ entry induced by cell depolarization triggered a transient Ca2+ release from the ER that was highly dependent on [Ca2+](ER ) and sensitized by low concentrations of caffeine. Caffeine-induced Ca2+ r elease was quantal in nature due to modulation by [Ca2+](ER). Whereas caffe ine released essentially all the Ca2+ from the ER, inositol 1,4,5-trisphosp hate (InsP(3))-producing agonists released only 60-80%. Both InsP(3) and ca ffeine emptied completely the ER in digitonin-permeabilized cells whereas c yclic ADP-ribose had no effect. Ryanodine induced permanent emptying of the Ca2+ stores in a use-dependent manner after activation by caffeine. Fast c onfocal [Ca2+], measurements showed that the wave of [Ca2+], induced by 100 -ms depolarizing pulses in voltage-clamped cells was delayed and reduced in intensity in ryanodine-treated cells. Our results indicate that the ER of chromaffin cells behaves mostly as a single homogeneous thapsigargin-sensit ive Ca2+ pool that can release Ca2+ both via InsP(3) receptors or CICR.