Rm. Seifar et al., Application of capillary electrochromatography to the separation of phenylthiohydantoin-amino acids, J CHROMAT A, 832(1-2), 1999, pp. 133-140
The applicability of capillary electrochromatography with 1.5-mu m ODS-modi
fied non-porous particles for the separation of phenylthiohydantoin (PTH)-a
mino acids was investigated. The effect of the pH, organic solvent content
and ion concentration of the mobile phase on the separation was investigate
d. On a 34-cm column, plate numbers in the order of 60 000-180 000 were obt
ained for the neutral and acidic PTH-amino acids. The required presence of
sodium dodecyl sulfate (SDS) in the mobile phase led to broad asymmetric pe
aks and long retention times for the three basic PTH-amino acids (Arg, His,
Lys). With a mobile phase containing a phosphate buffer with a pH of 7.2,
5 mM SDS, and 5% (v/v) of both acetonitrile and tetrahydrofuran, an isocrat
ic separation in less than 8 min could be realized for all PTH-amino acids
with the exception of PTH-Arg. (C) 1999 Published by Elsevier Science B.V.
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