Rapid quantification of indinavir in human plasma by high-performance liquid chromatography with ultraviolet detection

A rapid, sensitive and specific high-performance liquid chromatography (HPL C) procedure for the quantification of indinavir, a potent human immunodefi ciency virus (HN) protease inhibitor, in human plasma is described. Followi ng C-18 solid-phase extraction, indinavir was chromatographed on a reversed -phase C-8 column using a simple binary mobile phase of phosphate buffer-ac etonitrile (60:40, v/v). UV detection at 210 nm led to an adequate sensitiv ity without interference from endogenous matrix components. The limit of qu antification was 25 ng/ml with a 0.1 mi plasma sample. The standard curve w as linear across the range from 25 to 2500 ng/ml with an average recovery o f 91.4%. The mean relative standard deviations for concentrations within th e standard curve ranged between 1.4 and 9.7%. Quality control standards gav e satisfactory intra- and inter-assay precision (R.S.D. from 3.5 to 15.8%) and accuracy within 15% of the nominal concentration. Sample handling exper iments, including HN heat inactivation, demonstrated analyte stability unde r expected handling processes. The assay is suitable for the analysis of sa mples from adult and pediatric patients infected with Hn! (C) 1999 Elsevier Science B.V. All rights reserved.