p27(kip1) acts as a downstream effector of and is coexpressed with the beta(1C) integrin in prostatic adenocarcinoma

Integrins are a large family of transmembrane receptors that, in addition t o mediating cell adhesion, modulate cell proliferation. The beta(1C) integr in is an alternatively spliced variant of the beta(1) subfamily that contai ns a unique 48-amino acid sequence in its cytoplasmic domain. We have shown previously that in vitro beta(1C) inhibits cell proliferation and that in vivo beta(1C) is expressed in nonproliferative, differentiated epithelium a nd is selectively downregulated in prostatic adenocarcinoma. Here we show, by immunohistochemistry and immunoblotting analysis, that beta(1C) is coexp ressed in human prostate epithelial cells with the cell-cycle inhibitor p27 (kip1), the loss of which correlates with poor prognosis in prostate cancer . In the 37 specimens analyzed, beta(1C) and p27(kip1) are concurrently exp ressed in 93% of benign and 84%-91% of tumor prostate cells. Forced express ion of beta(1C) in vitro is accompanied by an increase in p27(kip1) levels, by inhibition of cyclin A-dependent kinase activity, and by increased asso ciation of p27(kip1) with cyclin A. beta(1C) inhibitory effect on cell prol iferation is completely prevented by p27(kip1) antisense, but not mismatch oligonucleotides. beta(1C) expression does not affect either cyclin A or E levels, or cyclin E-associated kinase activity, nor the mitogen-activated p rotein (MAP) kinase pathway. These findings show a unique mechanism of cell growth inhibition by integrins and point to beta(1C) as an upstream regula tor of p27(kip1) expression and, therefore, a potential target for tumor su ppression in prostate cancer.