Temporal and spatial expression of c-jun and jun-B proto-oncogenes in pulpcells involved with reparative dentinogenesis after cavity preparation of rat molars

c-jun and jun-B are nuclear proto-oncogenes induced by growth factors such as bone morphogenetic proteins (BMPs). These gene products enhance the expr ession of many genes, including osteocalcin and collagen types, indicating that c-jun and jun-B play important roles in the cell differentiation proce ss. It is also known that BMPs affect the differentiation of pulp cells to odontoblast-like cells during reparative dentinogenesis, but little is know n about the transcriptional regulation of genes in cells associated with re parative dentinogenesis. In this study, we examined the expression of c-jun and jun-B in pulp cells during reparative dentinogenesis after cavity prep aration of rat molars by in situ hybridization. In rat tooth germs, c-jun a nd jun-B were co-expressed in the odontoblastic lineage. In rat adult molar s, c-jun was expressed in the odontoblast layer, but the jun-B expression w as absent in all pulp cells. After cavity preparation, we found that c-jun and jun-B were coexpressed in pulp cells underneath cavities. During the ea rly phase of reparative dentinogenesis, levels of c-jun and jun-B greatly i ncreased in pulp cells within and around the reparative dentin matrix forme d adjacent to the cavity floor. Fourteen days after cavity preparation, c-j un and jun-B were expressed only in pulp cells lining the irregular surface of the thick reparative dentin. These results suggest that c-jun and jun-B may play important roles both in physiological acid in reparative dentinog enesis; in particular, the limited distribution of the jun-B expression sug gests a specific role of jun-B only in cells involved with the active forma tion of the dentin matrix during primary and reparative dentinogenesis.