High production of pyoluteorin and 2,4-diacetylphloroglucinol by Pseudomonas fluorescens S272 grown on ethanol as a sole carbon source

Pseudomonas fluorescens S272 newly isolated from a soil sample produced a c onsiderable amount of pyoluteorin and 2,4-diacetylphloroglucinol when grown on ethanol as a single carbon source, The coproduction of approximately 15 0 mu g/ml of pyoluteorin and approximately 500 mu g/ml of 2,4-diacyl-phloro glucinol was achieved by flask cultutivation in a medium containing approxi mately 2% ethanol. A high C/N ratio and inorganic phosphate limitation in t he medium were also important factors to be considered for optimization of production of the antibiotics.