Human effector memory T cells express CD86: A functional role in naive T cell priming

Citation
P. Jeannin et al., Human effector memory T cells express CD86: A functional role in naive T cell priming, J IMMUNOL, 162(4), 1999, pp. 2044-2048
Citations number
24
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
162
Issue
4
Year of publication
1999
Pages
2044 - 2048
Database
ISI
SICI code
0022-1767(19990215)162:4<2044:HEMTCE>2.0.ZU;2-V
Abstract
The glycoprotein CD86 expressed on APCs provides a costimulatory signal nec essary for an efficient activation of naive T cells. In contrast, there is controversy about the condition of expression and the function of CD86 on T cells, In this study, we have analyzed the phenotype and the biological ac tivity of CD86(+) T cells generated from human PBMC. Results show that CD86 expression on T cells is induced by long term stimulation via CD3 and IL-2 R and is down-regulated as the cells become quiescent. The CD86-expressing cells are memory effector T cells: 1) they express CD45RO and high levels o f the activation markers CD25, CD54, and HLA-Dr; 2) they selectively expres s CD30, CD40-ligand, and CD70; and 3) in response to stimulation, most of t hem produce IFN-gamma before dying by apoptosis. We then analyzed whether C D86 expressed on T cells is functional. Results show that paraformaldehyde- fixed CD86(+) T cells enhance the proliferation and production of IFN-gamma by anti-CD3 mAb-stimulated naive T cells and induce proliferation of resti ng allogenic T cells. All these effects are prevented by neutralizing anti- CD86 mAbs, In contrast, we report no autocrine effect of CD86 in CD86(+) T cell activation, In conclusion, these data show that human memory effector T cells express a functional form of CD86 that can costimulate naive T cell responses.