LOCALIZATION OF THE TRANSMEMBRANE-4 SUPERFAMILY (TM4SF) MEMBER PETA-3(CD151) IN NORMAL HUMAN TISSUES - COMPARISON WITH CD9, CD63, AND ALPHA-5-BETA-1 INTEGRIN

Citation
Pm. Sincock et al., LOCALIZATION OF THE TRANSMEMBRANE-4 SUPERFAMILY (TM4SF) MEMBER PETA-3(CD151) IN NORMAL HUMAN TISSUES - COMPARISON WITH CD9, CD63, AND ALPHA-5-BETA-1 INTEGRIN, The Journal of histochemistry and cytochemistry, 45(4), 1997, pp. 515-525
Citations number
39
Categorie Soggetti
Cell Biology
ISSN journal
00221554
Volume
45
Issue
4
Year of publication
1997
Pages
515 - 525
Database
ISI
SICI code
0022-1554(1997)45:4<515:LOTTS(>2.0.ZU;2-0
Abstract
It has recently been shown that several members of the tetraspan super family, including CD9 and CD63, associate with each other and with bet a 1 integrins. In this study, we examined the distribution of a recent ly identified tetraspan, PETA-3 (CD151), and of CD9, CD63, alpha 5 bet a 1, and the integrin beta 1 chain in normal human tissues by the indi rect immunoperoxidase and alkaline phosphatase-anti-alkaline phosphata se techniques. PETA-3 showed a broad distribution and was expressed by endothelium, epithelium, Schwann cells, and dendritic cells and by sk eletal, smooth, and cardiac muscle. Expression in skin was mostly rest ricted to the basal cells of the epidermis and was downregulated on di fferentiation. In the small intestine, PETA-3 was expressed by crypt a nd villous enterocytes with a mostly basolateral distribution, but was not detectable on the brush border. CD9 was expressed on the plasma m embrane of enterocytes in crypts and at the bases of the villi whereas CD63 demonstrated a unique granular appearance concentrated in the ap ical cytoplasm below the brush border. The findings of this study show co-localization of PETA3 with CD9, CD63, alpha 5 beta 1, and beta 1 i n particular tissues, demonstrating that tetraspan/integrin complexes may occur. However, the lack of co-localization of these antigens in o ther tissues also implies distinct roles for these molecules.