Simultaneous determination of hydrochlorothiazide and amiloride hydrochloride by ratio spectra derivative spectrophotometry and high-performance liquid chromatography

Rapid, precise, accurate and specific ratio spectra derivative spectrophoto metry and high-performance liquid chromatographic procedures were described for the simultaneous determination of hydrochlorothiazide and amiloride hy drochloride in combined pharmaceutical dosage forms. For the first method, ratio spectra derivative spectrophotometry, the signals were measured at 28 5.7 nm for hydrochlorothiazide and at 302.5 nm for amiloride hydrochloride in the mixture, in the first derivative of the ratio spectra. The second me thod is based on high-performance liquid chromatography (HPLC) on LiChrosor b RP-C-18 column (5 mu m, 20 cm x 4.6 mm) using 0.025 M orthophosphoric aci d (adjusted to pH 3.0 with triethylamine (TEA)), acetonitrile (84:16 v/v) a s a mobile phase at a how rate of 1.2 mi min(-1). Detection was carried out using a UV detector at 278.0 nm. Commercial sugar-coated and laboratory-pr epared mixtures containing both drugs in different proportions were assayed using the developed methods. (C) 1999 Elsevier Science B.V. All rights res erved.