Simultaneous determination of oxalic, fumaric, maleic and succinic acids in tartaric and malic acids for pharmaceutical use by ion-suppression reversed-phase high performance liquid chromatography

A reliable method for the simultaneous determination of oxalic, fumaric, ma leic, and succinic acids in tartaric and malic acids for pharmaceutical use by reversed-phase ion-suppression high performance liquid chromatography i s presented. HPLC was achieved on a Nova-Pak C-18 column by isocratic eluti on using water adjusted to pH 2.10-2.15 with perchloric acid, and detection was by UV adsorption at a wavelength of 210 nm. This method was found to b e superior to previous liquid chromatography as well as other classical ass ay, and to be an attractive choice for the analysis of these compounds. (C) 1999 Elsevier Science B.V. All rights reserved.