Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro

Authors
Okada, H Ohtsuka, H Kon-Nai, S Kirisawa, R Yokomizo, Y Yoshino, T Rosol, TJ
Citation
H. Okada et al., Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro, J VET MED S, 61(1), 1999, pp. 33-35
Citations number
16
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN journal
09167250 → ACNP
Volume
61
Issue
1
Year of publication
1999
Pages
33 - 35
Database
ISI
SICI code
0916-7250(199901)61:1<33:EOLOPO>2.0.ZU;2-B
Abstract
This investigation was performed to determine the effect of lipopolysacchar ide (LPS) on production of interleukin (IL)-1 and IL-6 by bovine mammary ep ithelial cells in vitro. After confluence, the cells were stimulated with L PS (0.1, 1.0 or 10 mu g/ml) for 4, 8, 24, and 48 hr. LPS increased producti on of both IL-1 and IL-6 production from mammary cells in a dose dependent manner. The expression of mRNA for IL-1 receptor antagonist (IL-1ra) was de monstrated by reverse transcription-polymerase chain reaction in bovine mam mary epithelial cells.