Sandwich enzyme-linked immunosorbent assay by using monoclonal antibody for detection of Clostridium perfringens enterotoxin

Authors
Piyankarage, RH Tajima, T Sugii, S Uemura, T
Citation
Rh. Piyankarage et al., Sandwich enzyme-linked immunosorbent assay by using monoclonal antibody for detection of Clostridium perfringens enterotoxin, J VET MED S, 61(1), 1999, pp. 45-47
Citations number
17
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN journal
09167250 → ACNP
Volume
61
Issue
1
Year of publication
1999
Pages
45 - 47
Database
ISI
SICI code
0916-7250(199901)61:1<45:SEIABU>2.0.ZU;2-Q
Abstract
Sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the qu antitative estimation of Clostridium perfringens enterotoxin (CPE) with mon oclonal and polyclonal antibodies as capturing and detecting antibodies, re spectively. The dose-dependent relationship between absorbance at 405 nm an d concentration of purified CPE was obtained over the range of 0.64-400 ng/ ml. The sandwich ELISA was found to detect crude CPE in culture and CPE in 10% fecal extracts. This method is convenient, rapid and sensitive for spec ific detection of CPE.