C. Warner et al., Use of anti-glycoprotein monoclonal antibodies to characterize rabies virus in formalin-fixed tissues, J VIROL MET, 77(1), 1999, pp. 69-74
Seventy anti-rabies virus monoclonal antibodies (Mabs) were tested for reac
tivity with rabies and rabies-related viruses in formalin-fixed (FF) tissue
s. Forty-three of the Mabs were directed against the glycoprotein and 27 we
re directed against the nucleocapsid as determined by enzyme immunoassays a
nd neutralization tests. Twenty of the anti-glycoprotein Mabs and one of th
e anti-nucleocapsid Mabs reacted with the rabies challenge virus strain (CV
S) in FF tissue. These 21 Mabs were screened against other lyssaviruses in
FF tissues: five rabies virus strains (coyote, skunk, raccoon, red bat, and
silver-haired bat), and four rabies-related viruses (Australian bat lyssav
irus, Duvenhage virus, Lagos bat virus, and Mokola virus). One of the anti-
glycoprotein Mabs was reactive with all the virus strains screened. Another
of the anti-glycoprotein Mabs reacted with all of the rabies virus strains
tested, but not with any of the rabies-related virus strains tested. The r
emaining Mabs had reactivity patterns that could be useful for characterizi
ng lyssaviruses in FF tissues. (C) 1999 Elsevier Science B.V. All rights re
served.