Purification and monolayer study of the thylacoid lipids of moss Marchantia polymorpha

The original lipid content of the thylakoid membranes of moss Marchantia po lymorpha has been determined for the first time. In particular, the content of SQDG is almost 3 times higher than those for both of the other classes. The ratios for DGDG and MGDG are just a little bit lower than those for gr een algae, but almost 2 times less than those for plants. The distribution of unsaturated bonds has changed in C-18 -residues of fatty acids. The tota l content of C-18-residues in thylacoid lipids have been almost the same bu t the content of C-18:1 , C-18:2 and C-18:3 are altered in the fractions of DGDG, PG and PE. The light stress produces only the quantitative, but no q ualitative, changes of the thylakoid lipid composition. The properties of t he thylakoid lipids and corresponding fatty acids in the monolayers at the liquid/gas interfaces have been studied. The changes in distribution of uns aturated bonds in C-18 residues of fatty acids at light stress have been co nfirmed by Langmuir method.