Endosulfan, an organochlorine pesticide, was subjected to the differential
sensitivity assay in repair-deficient and repair-proficient strains of Esch
erichia coli K12, prophage lambda induction assay in WP2s (lambda) and muta
tion induction in E. coli K12. The induction of umu gene expression with en
dosulfan was studied also in Salmonella typhimurium TA1535/pSK1002 cells. T
he differential sensitivity assay revealed that the recA 13 strain was the
most sensitive. Endosulfan induced prophage lambda in E. coli and umu gene
expression in S. typhimurium cells; however, the extent of the effects were
low. Endosulfan also induced a dose-dependent increase in forward mutation
s in E. coli K12 cells from ampicillin sensitivity to ampicillin resistance
. Our studies indicate the genotoxic potential of endosulfan and the role o
f the recA gene in the repair of endosulfan-induced DNA damage. (C) 1999 El
sevier Science B.V. All rights reserved.