D. Otto, SOME PROPERTIES OF THE FEMALE SEX-PHEROMONE OF THE COLORADO POTATO BEETLE LEPTINOTARSA-DECEMLINEATA SAY (COL CHRYSOMELIDAE), Anzeiger fur Schadlingskunde, Pflanzenschutz, Umweltschutz, 70(2), 1997, pp. 30-33
The female sex pheromone of L. decemlineata is an aphrodisiacum, switc
hing on mating behaviour in contacting males. A biotest is described t
o estimate quantitatively the exciting power of a subject (female, iso
lated elytrae, glass dummy coated with extract) to males. The pheromon
e is present only in fertile females, also in freshly killed ones. It
can be extracted by organic solvents. A dummy imitating a beetle's abd
omen and contaminated with the residue of a female s wash solution is
exciting to males too. Suitable solvents are ethylalcohol, acetonet, h
exane. By this, the pheromone of 0,1 female is detectable. The residue
can be taken up again with the same or an other solvent. The solution
can be stored at -18 degrees C without significant losses, but at tem
peratures >+5 degrees C their efficacy diminishes during 2 weeks. The
exciting efficacy of the dry residue desreases to about 50% during nea
rly 4 weeks (at +22 degrees C) or 9 weeks (at +5 degrees C). The phero
mone resists heating to about +160 degrees C and is destroyed at highe
r temperatures.