EFFECT OF LIPOSOME-ENCAPSULATED HEMOGLOBIN ON TRIGLYCERIDE, TOTAL CHOLESTEROL, LOW-DENSITY-LIPOPROTEIN, AND HIGH-DENSITY-LIPOPROTEIN CHOLESTEROL MEASUREMENTS

The present study investigated the effect of liposome-encapsulated hem oglobin (LEH), an experimental oxygen-carrying resuscitation fluid, on triglyceride, total cholesterol, and low density lipoprotein (LDL), a nd high density lipoprotein (HDL) cholesterol measurements. In vivo, t he intravenous infusion of LEH (5.6 mL/kg, n = 6) elevated serum trigl ycerides (+ 92% vs. baseline, P < .05), total cholesterol (+ 25% vs. b aseline, P < .01), LDL cholesterol (+ 72% vs. base line, P < .01) and had no effect on serum HDL cholesterol. In addition, LEH did not alter the elevation in serum triglycerides (+ 302% vs. baseline, P < .01) a nd LDL cholesterol (+ 86% vs. baseline, P < .01) induced by lipopolysa ccharide (3.6 mg/kg, i.v., n = 6). Ex vivo, measurements of triglyceri des and total cholesterol as well as LDL and HDL cholesterol in whole blood from naive rats were not changed by the addition of LEH (0-50%, n = 6). In vitro, the addition of a fixed concentration of LEH (50%, n = 6) to varying concentrations of cholesterol solution (0-50%), or vi ce versa, had no effect on cholesterol determination. It is therefore concluded that LEH only minimally affects serum levels of triglyceride s, total cholesterol, LDL cholesterol, and HDL cholesterol and does no t interfere with their measurement.