Translocation of chicken heart apocytochrome c and its mutants (C17S, H18D) across mitochondrial membrane

Authors
Zhu, Y Han, XH Yang, FY
Citation
Y. Zhu et al., Translocation of chicken heart apocytochrome c and its mutants (C17S, H18D) across mitochondrial membrane, SCI CHINA C, 42(1), 1999, pp. 1-7
Citations number
22
Categorie Soggetti
Experimental Biology
Journal title
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN journal
10069305 → ACNP
Volume
42
Issue
1
Year of publication
1999
Pages
1 - 7
Database
ISI
SICI code
1006-9305(199902)42:1<1:TOCHAC>2.0.ZU;2-B
Abstract
The dependence of import of chicken heart apocytochrome c on its transforma tion to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitoch ondrial membrane in the presence or absence oi hemin + dithionite. Furtherm ore, two heme unattached mutants (H18D, C17S) were prepared, which could st ill be accumulated in mitochondria, bur their import velocity was obviously reduced.