Determination of carboplatinum and cisplatinum by interfacing HPLC with ICP-MS using ultrasonic nebulisation

A novel HPLC technique has been developed for the determination of carbopla tinum(II) and cisplatinum(II) which are used for chemotherapy. The compound s were separated by a HPLC column. The eluent was on-line nebulized into th e nebuliser gas flow using ultrasonic nebulisation. The resulting aerosol w as driven by the nebuliser gas stream through a 30-cm heating path, a 60-cm cooling path for desolvatisation and into the plasma of the ICP-MS. The de tection was carried out on platinum mass Pt-195. Different RP C18 technique s were tested to obtain the best separation and detection results of the tw o platinum compounds. The solvent-generated anion exchanger method was sele cted as the method with the best handling and sensitivity. This technique a llows a good separation of the two compounds within 3 min. The detection li mits for carboplatinum and cisplatinum were 130 and 80 pg, respectively. Th e newly developed system has been applied to study the behaviour of the pla tinum species under different anion influences in aqueous solutions. These investigations are necessary for the future development of an extraction fr om environmental samples for the two compounds. In further experiments the distribution of the two platinum species, Na2Pt(II)Cl-4 and the palladium s pecies Na2Pd(II)Cl-4 between water/sediment, water/charcoal, sewage sludge/ water and the distribution in the solution after iron flocculation were inv estigated to plan an extraction method for the platinum species from enviro nmental matrices. (C) 1999 Elsevier Science B.V. Ail rights reserved.