The behaviour of various surfactants and other detergent components in biod
egradation tests was studied in art extensive research project. The biodegr
adation tests were carried out in laboratory- and pilot-scale activated slu
dge plants The operating parameters of these plants correspond to modern fu
ll-scale waste water treatment plants and include nitrification and pre-den
itrification. Specific analytical methods, based mainly on HPLC, were devel
oped to determine the biodegradation of the investigated surfactants and ot
her detergent components. The equipment and analytical methods used are des
cribed here and typical chromatograms are shown.