Zh. Xiao et al., Modulation of platelet-neutrophil interaction with pharmacological inhibition of fibrinogen binding to platelet GPIIb/IIIa receptor, THROMB HAEM, 81(2), 1999, pp. 281-285
The study investigated how drug inhibition of the GPIIb/IIIa receptor influ
ences the interactions between platelets and leukocytes. These interactions
are believed to play an important role in the etiology of the acute corona
ry syndromes. Thirty patients with unstable angina or non-Q-wave myocardial
infarction were studied before the administration of tirofiban or placebo
and after 4 h and 72 h, Platelet-leukocyte aggregates were characterized in
whole blood using three-colour flow cytometry. The leukocyte population wa
s isolated by a nucleic acid probe (LDS 751) and platelet-neutrophil coaggr
egates identified as particles binding both anti-CD42a-FITC and anti-CD45-P
E. Tirofiban decreased by 25% the density of platelets in circulating plate
let-neutrophil coaggregates (p <0.01), and prevented the increase induced b
y platelet agonist stimulation (p <0.0001). The reduction correlated with i
nhibition of fibrinogen binding to platelet (p <0.0001) and with inhibition
of platelet aggregation (p < 0.0001). The percentage of neutrophils with b
ound platelets following platelet agonist stimulation was, however, increas
ed following GPIIb/IIIa inhibition. Thus, inhibition of GPIIb/IIIa receptor
promotes platelet-neutrophil adhesion, but markedly reduces the binding de
nsity of platelets in the coaggregates.