Intranasal or subcutaneous co-administration of recombinant cholera toxin B subunit stimulates only a slight or no level or the specific IgE responsein mice to tetanus toxoid

Whether recombinant cholera toxin B subunit (rCTB) co-administered intranas ally or subcutaneously with aluminium-nonadsorbed tetanus toroid (nTT) can induce the production of tetanus toroid mi-specific IgE antibodies in mice was investigated compared with aluminium-adsorbed tetanus toroid (aTT) admi nistered intranasally of subcutaneously. Mice immunized intranasally or sub cutaneously with nTT together with rCTB showed a high level of TT-specific serum IBG antibody response and no or a slight level of TT-specific serum I gE antibody response. On the other hand, in mice vaccinated intranasally or subcutaneously with aTT alone. higher levels of TT-specific IgG and IgE an tibodies were induced in comparison with intranasal or subcutaneous inocula tion of nTT together with rCTB. These results suggest that intranasal or su bcutaneous coadministration of rCTB with nTT is better than intranasal or s ubcutaneous administration or aTT to avoid IgE-mediated allergic reactions. (C) 1999 Elsevier Science Ltd. All rights reserved.