A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease

Authors
Padmanabhan, P Shanker, R Khanna, P
Citation
P. Padmanabhan et al., A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease, WORLD J MIC, 14(6), 1998, pp. 925-926
Citations number
3
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993 → ACNP
Volume
14
Issue
6
Year of publication
1998
Pages
925 - 926
Database
ISI
SICI code
0959-3993(199811)14:6<925:AMFEOD>2.0.ZU;2-Y
Abstract
A protocol for extraction of microbial DNA from oil-and grease-saturated so il is reported that can be used to target availability of specific genotype s of bacteria in extensively contaminated soils using the polymerase chain reaction. The methodology developed can be used in the biotreatibility stud ies for in situ bioremediation of hydrocarbon-contaminated sites.