N. Das et Am. Kayastha, Immobilization of urease from pigeonpea (Cajanus cajan L.) on flannel cloth using polyethyleneimine, WORLD J MIC, 14(6), 1998, pp. 927-929
Urease of pigeonpea has been immobilized on polyethyleneimine-activated cot
ton cloth followed by cross-linking with dimethyl suberimidate. Optimum imm
obilization (56%) was obtained at a protein loading of 1.2 mg/5 x 5 cm(2) c
loth piece. The immobilized enzyme stored in 0.1 IM Tris/acetate buffer, pH
6.5, at 4 degrees C had a t(1/2) of 70 days. There was practically no leac
hing of the enzyme from the immobilization matrix in 15 days. The immobiliz
ed enzyme was used 7 times at an interval of 24 h between each use with 75%
residual activity at the end of the period. Blood urea analysis was carrie
d out with immobilized urease for some clinical samples.