Citation
N. Jothikumar et al., Membrane-impregnated probe for simultaneous PCR amplification and detection, WORLD J MIC, 14(6), 1998, pp. 933-934
Citations number
3
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993
→ ACNP
Volume
14
Issue
6
Year of publication
1998
Pages
933 - 934
Database
ISI
SICI code
0959-3993(199811)14:6<933:MPFSPA>2.0.ZU;2-F
Abstract
We report a rapid and highly versatile one-step single tube method for gene
amplification and detection using a non-radiolabelled probe. The technique
requires nylon on which a third primer has been immobilized that acts as a
probe and digoxigenin-labelled-dUTP added to the conventional PCR mixture.
After PCR amplification the membrane-impregnated probe is labelled with di
g-11-dUTP that serves to confirm the identity of the PCR product.