Plasma amino acid levels after carbon tetrachloride induced acute liver damage. A dose-response and time-response study in rats

Citation
M. Holecek et al., Plasma amino acid levels after carbon tetrachloride induced acute liver damage. A dose-response and time-response study in rats, AMINO ACIDS, 16(1), 1999, pp. 1-11
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
AMINO ACIDS
ISSN journal
09394451 → ACNP
Volume
16
Issue
1
Year of publication
1999
Pages
1 - 11
Database
ISI
SICI code
0939-4451(1999)16:1<1:PAALAC>2.0.ZU;2-N
Abstract
The aims of the present study were to assess the changes of individual plas ma amino acid levels in relation (1) to the severity of liver damage and (2 ) to the process of liver recovery. Acute liver injury was induced by an in tragastric administration of CCl4 diluted in olive oil in doses of 2, 4 and /or 6g of CCl4 per kg b.w. The control rats received olive oil only. Animal s were sacrificed at 16, 24, 48 and 96 hours after treatment. The severity of liver injury was assessed by histological examination, by changes in ALT and AST in the blood plasma and by changes in liver weight. Statistical an alysis was carried by ANOVA, p < 0.05 was considered significant. The Spear man rank correlation coefficient was used as a measure of the degree of lin ear relationship between variable and dose. In the period of the developmen t of acute liver damage, i.e. at 16 and 24 hours after treatment, an increa se in blood plasma amino acid levels and positive correlations with the dos e of CCl4 were observed for most individual amino acids. The only exception was arginine which decreased in a dose dependent manner. At a phase of liv er recovery, i.e. at 48 and 96 hours after CCl4 treatment, the concentratio ns of some individual amino acids decreased below the control values. The n egative correlation with the dose of CCl4 occurred for taurine and isoleuci ne (at 48 hours) and taurine, threonine, valine? methionine, isoleucine and leucine (at 96 hours).