Mapping of the Na+, K+-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes in pig by somatic cell hybrid analysis

Two expressed sequence tags were isolated from a porcine skeletal muscle cD NA library and identified as the putative partial cDNAs of the porcine Na+, K+-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) g enes after sequencing and homology search. Results of analysis of a pig-rod ent somatic cell hybrid panel by PCR allowed the assignments of A TP1A2 to porcine chromosome (chr) 4 and of PFKM to porcine chr 5. These assignments support previously observed conservation of syntenic relationships between human chr 1 and porcine chr 4 and between human chr 12 and porcine chr 5.