R. Bermudez-cruz et al., Characterization of a bovine herpesvirus 4 (BHV-4) 1.1-kb RNA and its transactivation by BHV-4 immediate-early 2 gene product, ARCH VIROL, 143(12), 1998, pp. 2391-2412
We determined the structure of a 1.1-kb cytoplasmic polyadenylated RNA tran
scribed from a region of the bovine herpesvirus 4 (BHV-4) genome not conser
ved among gammaherpesviruses by sequencing its cDNA, by SI nuclease analysi
s, and by primer extension analysis. We found that the RNA consists of a sh
ort, approximately 193-nucleotide (nt), 5' exon spliced to a 799-nt 3' exon
and contains two short (53 and 57 codons) overlapping open reading frames
(ORFs). The 53-codon ORF was previously designated BORFE1. Neither ORF exhi
bits detectable amino acid sequence homology with ORFs of other gammaherpes
viruses. The 1.1-kb RNA promoter-regulatory region was specifically transac
tivated by the BHV-4 IE2 gene product, a homolog, of the Epstein-Barr virus
R transactivator, in cotransfection assays. In gel retardation experiments
, IE2 protein formed a complex with DNA in a 129-bp fragment between -23 an
d -151 relative to the transcription start site of the 1.1-kb RNA, and less
efficiently with a 57-bp subfragment between -78 and -22. A sequence simil
ar to sequences of IE2-binding fragments of other BHV-4 IE2 responsive prom
oters was found partly in the 57-bp subfragment, extending into the portion
of the 129-bp fragment not found in the 57-bp fragment. The 129-bp fragmen
t, but not the 57-bp fragment, was sufficient for transactivation of a prom
oterless chloramphenicol acetyl transferase (CAT) reporter gene by IE2. How
ever, the 129-bp fragment did not function efficiently as an IE2-responsive
enhancer when inserted approximately 140 base pairs (bp) 5/ to the transcr
iption start site of a CAT reporter gene driven by an enhancerless simian v
irus 40 early promoter. Based on this and other observations, we propose th
at IE2 functions as a promoter factor rather than an enhancer factor.