C. Pecqueur et al., Functional organization of the human uncoupling protein-2 gene, and juxtaposition to the uncoupling protein-3 gene, BIOC BIOP R, 255(1), 1999, pp. 40-46
Citations number
36
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Human and mouse UCP2 genes were cloned and sequenced. Transcriptional start
sites were identified using primer extension analysis. The transcription u
nit of UCP2 gene is made of 2 untranslated exons followed by 6 exons encodi
ng UCP2. In vitro translation analysis demonstrated that an open-reading-fr
ame for a putative peptide of 36 residues present in exon 2 did not prevent
UCP2 translation and confirmed that the initiation site of translation was
in exon 3 as predicted from sequencing data. Short (bp -125 to +93) and lo
ng (bp -1383 and +93) CAT-constructs containing DNA upstream of the transcr
iptional start site of the human gene were made and transfected in adipocyt
es or HeLa cells allowing characterization of a potent promoter. Analysis o
f several genomic clones encompassing UCP2 and/or UCP3 genes demonstrated t
hat the 2 genes are adjacent, the human UCP2 gene being located 7 kb downst
ream of the UCP3 gene. (C) 1999 Academic Press.