The NAD(+) precursors, nicotinic acid and nicotinamide upregulate glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase mRNAin Jurkat cells

To better understand the role of nicotinic acid and nicotinamide in the reg ulation of the oxidative stress response, we measured the levels of glycera ldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydroge nase (G6PD) mRNA in Jurkat cells treated with these NAD(+) precursors. We u sed a modified nonradioactive Northern blot method and detected the mRNA us ing 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observe d increased levels of the mRNAs for the two enzymes in treated cells. Our f indings suggest that the NAD(+) precursors may protect against oxidative st ress and DNA damage by up-regulating the stress response genes GAPDH and G6 PD. (C) 1999 Academic Press.