Transgenie tobacco and potato plants expressing basic vacuolar beta-1,3-glucanase from Nicotiana plumbaginifolia

Transgenic tobacco and potato plants constitutively overexpressing beta-1,3 -glucanase gene from Nicotiana plumbaginifolia were prepared under the cont rol of the cauliflower mosaic virus (CaMV) 35S promoter. The introduction o f N. plumbaginifolia beta-1,3-glucanase gene into the tobacco and potato ge nome was proven by Southern blot analysis, and the expression of beta-1,3-g lucanase was detected by Western blotting. The crude protein extracts from transgenic potato plants were analysed by native cathodic electrophoresis, using laminarin as a substrate. These plants will be used in resistance ass ays against Rhizoctonia solani and Fusarium oxysporum in greenhouse tests.