Determination of platinum in rat dorsal root ganglion using ICP-MS

This study evaluated the performance of inductively coupled plasma mass spe ctrometry for the determination of platinum (Pt) in rat dorsal root ganglio n. The method detection limit was found to be 0.008 ng/mL of Pt, which corr esponds to 4 pg of Pt per milligram of ganglia. The standard deviations in the tissue matrix were 5.7% or better and minimum matrix effect was observe d. Compared to indium, the use of iridium or a combination of iridium and b ismuth as internal standard(s) provided more accurate measurement. The Pt i n the tissue digestate was stable for a minimum of 46 d at levels above 0.0 5 ng/mL. Flow injection analysis using undiluted digestates resulted in app roximately 20% signal enhancement. Internal standard correction was necessa ry to obtain accurate results. The method was used in initial studies in wh ich rats were dosed with cisplatin and has shown that Pt accumulates and pe rsists in dorsal rat ganglion following treatment.