Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly(carbamoyl) sulfonate (PCS) part I: Construction and characterization of the microbial sensor

Authors
Chan, CY Lehmann, M Tag, K Lung, M Kunze, G Riedel, K Gruendig, B Renneberg, R
Citation
Cy. Chan et al., Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly(carbamoyl) sulfonate (PCS) part I: Construction and characterization of the microbial sensor, BIOSENS BIO, 14(2), 1999, pp. 131-138
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOSENSORS & BIOELECTRONICS
ISSN journal
09565663 → ACNP
Volume
14
Issue
2
Year of publication
1999
Pages
131 - 138
Database
ISI
SICI code
0956-5663(19990201)14:2<131:MOBSUT>2.0.ZU;2-I
Abstract
A microbial biosensor based on the yeast Arxula adeninivorans LS3 has been developed for measurement of biodegradable substances. Arxula is immobilize d in the hydrogel poly(carbamoyl) sulfonate (PCS). The immobilized yeast me mbrane is placed in front of an oxygen electrode with - 600 mV versus Ag/Ag Cl. Arxula is salt tolerant; it can give a stable signal up to 2.5 M NaCl i n sample (120 mM in measuring cell). The sensor's measurements are highly c orrelated to BOD5 measurements. It has a very high stability which can last for 40 days without any decrease in signal. The linear range of the sensor is up to a corresponding BOD value of 550 mg/l. (C) 1999 Elsevier Science S.A. All rights reserved.