Determination of the breakpoint of the common alpha-thalassaemia deletion in Filipinos in Hawaii

Authors
Hunt, JA Lee, L Donlon, TA Hsia, YE
Citation
Ja. Hunt et al., Determination of the breakpoint of the common alpha-thalassaemia deletion in Filipinos in Hawaii, BR J HAEM, 104(2), 1999, pp. 284-287
Citations number
8
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
104
Issue
2
Year of publication
1999
Pages
284 - 287
Database
ISI
SICI code
0007-1048(199902)104:2<284:DOTBOT>2.0.ZU;2-J
Abstract
The region of the crossover causing the Filipino type of alpha thalassaemia has been determined by examining similarity between the regions which had been indicated as involved in the crossover points by restriction mapping, using the published alpha-globin region DNA sequence, The crossover point w as found in 21 base pairs between two Alu sequences using PCR primers flank ing these Alu sequences. A simple PCR multiplex assay has been devised to d etect heterozygotes and homozygotes.